Search for: All records

Immune cells have intensely physical lifestyles characterized by structural plasticity and force exertion. To investigate whether specific immune functions require stereotyped mechanical outputs, we used super-resolution traction force microscopy to compare the immune synapses formed by cytotoxic T cells with contacts formed by other T cell subsets and by macrophages. T cell synapses were globally compressive, which was fundamentally different from the pulling and pinching associated with macrophage phagocytosis. Spectral decomposition of force exertion patterns from each cell type linked cytotoxicity to compressive strength, local protrusiveness, and the induction of complex, asymmetric topography. These features were validated as cytotoxic drivers by genetic disruption of cytoskeletal regulators, live imaging of synaptic secretion, and in silico analysis of interfacial distortion. Synapse architecture and force exertion were sensitive to target stiffness and size, suggesting that the mechanical potentiation of killing is biophysically adaptive. We conclude that cellular cytotoxicity and, by implication, other effector responses are supported by specialized patterns of efferent force. 

This paper presents a novel physical gripping framework intended for controlled, high force density attachment on a range of surfaces. Our framework utilizes a light-activated chemical adhesive to attach to surfaces. The cured adhesive is part of a "sacrificial layer," which is shed when the gripper separates from the surface. In order to control adhesive behavior we utilize ultraviolet (UV) light sensitive acrylics which are capable of rapid curing when activated with 380nm light. Once cured, zero input power is needed to hold load. Thin plastic parts can be used as the sacrificial layers, and these can be released using an electric motor. This new gripping framework including the curing load capacity, adhesive deposition, and sacrificial methods are described in detail. Two proof-of concept prototypes are designed, built, and tested. The experimental results illustrate the response time (15-75s depending on load), high holding force-to-weight ratio (10-30), and robustness to material type. Additionally, two drawbacks of this design are discussed: corruption of the gripped surface and a limited number of layers.